Part:BBa_K2888007:Design

fused chimerical gene (lysozyme and mlrA)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1349
  Illegal AgeI site found at 884
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We try to link two separate gene together

Source

We use common, standard promoter, RBS and terminator. This mlrA gene is a part of the microcystin degradation system discovered in Sphingopyxis sp. C-1 and the cyanophage lysozyme was discovered by Heideburg et al in Yellowstone National Park {Heideburg:2009hb}